Results Figure 1a: LB ampicillin plate with BL21(DE3) E. coli bacteria and pGEM-gbr22 plasmid incubated in SOC media.
Figure 1b: LB ampicillin plate with BL21(DE3) E. coli bacteria incubated in SOC media without any plasmid. Figure 1c: LB plate without ampicillin. Bacteria sample was taken from a sink handle in the lab. Figure 2: The incubated flasks of LB and 100ug/ml ampicillin with the transformed E. coli bacteria, turned purple from the production of the protein being expressed. Figure 3: The remaining pellets after centrifugation and after the remaining liquid was disposed of. Figure 4: PAGE gel of 6 samples from left to right: Lysate, Soluble fraction of the pellet, Flow, 20mM imidazole Wash, Elution 1, and Elution 2 from previous lab.
Introduction:
Materials & Methods:
Results
Figure 1a: LB ampicillin plate with BL21(DE3) E. coli bacteria and pGEM-gbr22 plasmid incubated in SOC media.
Figure 1b: LB ampicillin plate with BL21(DE3) E. coli bacteria incubated in SOC media without any plasmid.
Figure 1c: LB plate without ampicillin. Bacteria sample was taken from a sink handle in the lab.
Figure 2: The incubated flasks of LB and 100ug/ml ampicillin with the transformed E. coli bacteria, turned purple from the production of the protein being expressed.
Figure 3: The remaining pellets after centrifugation and after the remaining liquid was disposed of.
Figure 4: PAGE gel of 6 samples from left to right: Lysate, Soluble fraction of the pellet, Flow, 20mM imidazole Wash, Elution 1, and Elution 2 from previous lab.
Discussion:
Conclusions:
References: